Research Specialist - Biochemistry

Location

ST. LOUIS, MO 63110

Scheduled Hours

40

Position Summary

The Ghanbarpour Laboratory in the Department of Biochemistry and Molecular Biophysics at WashU Medicine is seeking a highly motivated Research Specialist to join our interdisciplinary research program.

Our lab investigates how bacteria and human mitochondria maintain homeostasis and respond to stress and environmental challenges. A central focus of our research is uncovering the molecular mechanisms of ATP-dependent AAA+ proteolytic machines, both soluble and membrane-spanning, and their accessory factors in bacterial and mitochondrial systems. We study how these complexes assemble, achieve substrate specificity, and promote bacterial recovery from antibiotic stress, environmental changes, and pathogen attack. These efforts integrate cryo-EM and cryo-ET structural biology, biochemical and cellular assays, and mass spectrometry.

The lab also explores bacterial defense mechanisms against bacteriophage infection, including the deployment of innate immune strategies that enable survival during phage attack. Another major focus of our research is protein engineering and the development of new molecular tools based on these systems.

In this role, you will receive extensive hands-on training in cryo-EM sample preparation and data analysis, heterogeneity analysis tools, mass spectrometry, membrane biology, biochemistry, and cellular assays. The Ghanbarpour Laboratory and WashU Medicine provide state-of-the-art infrastructure for structural biology, biochemistry, and mass spectrometry, along with a highly collaborative research environment.

Provides critically complex research and experimentation for department/s allowing the advancement of scientific analysis and discovery. Serves as a subject matter expert offering support, training and guidance relative to the discipline/area. May participate and or present on the research developed, or the established work of those within the department.

The position is supported by NIH grant R35GM160328, the Longer Life Foundation, the Siteman Cancer Center Catalysts Award, and Washington University startup funds.

Related papers

1.     Zhang T.; Lyu, Y.; Beck, C.; Iqbal, N.; Barbosa R.; Ghanbarpour, A.; Laub, T. M.; Activation of a bacterial immunity protein via supramolecular assembly with multiple phage triggers, Nature (2026).

2.     Iqbal, N.; Keller, S.; Ghanbarpour, A. Structural plasticity of the membrane-bound protein degradation assembly supports bacterial adaptation to stress. Cell Reports, 2026.

3.     Ghanbarpour, A.; Telusma, B.; Powell, B.; Zhang, J. J.; Bolstad, I.; Vargas, C.; Keller, S.; Baker, T. A; Sauer, R. T; Davis, J. H.; An asymmetric nautilus-like HflK/C assembly controls FtsH proteolysis of membrane proteins, The EMBO Journal, 44, 2501 (2025).

4.     Ghanbarpour, A.; Sauer, R. T; Davis, J. H.;  A proteolytic AAA+ machine poised to unfold a protein substrate, Nature Communications, 15, 9681 (2024).

Job Description

Primary Duties & Responsibilities

• Performs complex analyses, experiments and project work under general direction of the PI. Serves as representative for PI and ensures procedures and protocols are adhered to consistently and effectively meet department's standard. Exhibits a high degree of independent problem solving and ability to conduct research on assigned projects. May serve in lead role as needed.
• Plans, develops, organizes and formalizes research results and findings. May develop for presentation and/or present on behalf of department. Conducts literature searches related to research projects. Writes/co-authors publications and/or papers supporting research findings in logical and sometimes multiple formats tailored to audiences. May participate in work related to grants.
• Solves practical problems relating to difficulty with equipment or test subjects. Suggests technical/ procedural improvements in various methods. May develop and optimize experimental procedures and protocols. Provides high-level troubleshooting to advance process or lab procedures.
• Serves as department's research representative and may participate in various related meetings and events. Gathers/delivers data/information and serves as subject matter expert. Performs statistical data analysis, as needed. Presents research information to internal and/or external audiences.
• Organizes and maintains required documentation on lab and/or specimen conditions and can develop and summarize information into usable formats or presentations on behalf of the department. May communicate findings to various users or external audiences.
• Performs all related work in accordance with established protocols and safety/compliance requirements.
• May explain methods, lead or train other personnel or observers.

Working Conditions

• Works in a laboratory environment with potential exposure to biological and chemical hazards.
• Must be physically able to wear protective equipment and to provide standard care to research animals.

The above statements are intended to describe the general nature and level of work performed by people assigned to this classification. They are not intended to be construed as an exhaustive list of all job duties performed by the personnel so classified. Management reserves the right to revise or amend duties at any time.

Required Qualifications

Education

Master’s degree or combination of education and/or experience may substitute for minimum education.

Certifications /Professional Licenses

No specific certification/professional license is required for this position.

Work Experience

Laboratory Setting (4 Years)

Skills

Not Applicable

Driver's License

A driver's license is not required for this position.

More About This Job

Preferred Qualifications

• Strong background in protein purification and biochemical assays is highly preferred.
• Experience in biochemistry, microbial genetics, cell biology, or protein engineering.

Preferred Qualifications

Education

No additional education unless stated elsewhere in the job posting.

Certifications /Professional Licenses

No additional certification/professional licenses unless stated elsewhere in the job posting.

Work Experience

No additional work experience unless stated elsewhere in the job posting.

Skills

Critical Thinking, Detail-Oriented, Laboratory Instrumentation, Laboratory Research, Oral Communications, Recordkeeping, Self-Starter, Software Program, Spreadsheets, Statistical Software, Written Communication

Grade

R09

Salary Range

Base pay is commensurate with experience.

The salary range reflects base salaries paid for positions in a given job grade across the University. Individual rates within the range will be determined by factors including one's qualifications and performance, equity with others in the department, market rates for positions within the same grade and department budget.

Questions

For frequently asked questions about the application process, please refer to our External Applicant FAQ .

Accommodation

If you are unable to use our online application system and would like an accommodation, please email   CandidateQuestions@wustl.edu   or call the dedicated accommodation inquiry number at 314-935-1149 and leave a voicemail with the nature of your request.

All qualified individuals must be able to perform the essential functions of the position satisfactorily and, if requested, reasonable accommodations will be made to enable employees with disabilities to perform the essential functions of their job, absent undue hardship.

Pre-Employment Screening